The combination of nucleic acid extraction, gene amplification, nucleic acid probe hybridization and immunochromatographic technology to target microbial nucleic acid target sequence of highly conserved regions, including specific primers and probe labeled nucleic acid in the reaction system, complete the expansion of 37 DEG C under the condition of constant temperature increase, then the amplified products were inserted into the reaction tube anti pollution leak proof test card, not open cover Immunostrip chromatography qualitative determination. The method is free from expensive detection equipment and is suitable for the rapid detection of genes in the primary laboratory.

The advantage of this technology lies in:

■ In response to the special fast, short reaction time.

■ In operation, a reaction tube only two times to add.

■ - products higher detection sensitivity, the detection sensitivity is higher than that of the qPCR reaction.

This platform is mainly used in bacterial detection, animal and animal disease detection (Mycoplasma bovis, canine distemper, canine parvovirus, and small ruminant brucellosis) detection.